{"product_id":"elk-elk5146-human-stat5b-signal-transducer-and-activator-of-transcription-5b-elisa-kit","title":"Human STAT5B(Signal Transducer And Activator Of Transcription 5B) ELISA Kit","description":"\u003cp\u003eHuman STAT5B(Signal Transducer And Activator Of Transcription 5B) ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of STAT5B.\u003c\/p\u003e\u003ch3\u003eTest principle\u003c\/h3\u003e\u003cp\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human STAT5B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human STAT5B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human STAT5B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human STAT5B in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\u003ch3\u003eTarget background\u003c\/h3\u003e\u003cp\u003eAlternative names include STAT5-B.\u003c\/p\u003e","brand":"ELK Biotechnology","offers":[{"title":"48T","offer_id":56181862105428,"sku":"ABM69248","price":236.41,"currency_code":"GBP","in_stock":true},{"title":"96T","offer_id":56181862138196,"sku":"ABM69247","price":334.17,"currency_code":"GBP","in_stock":true},{"title":"96T*5","offer_id":56181862170964,"sku":"ABM69249","price":1420.22,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/ELK5146-b-1.jpg?v=1781996032","url":"https:\/\/www.abmium.com\/products\/elk-elk5146-human-stat5b-signal-transducer-and-activator-of-transcription-5b-elisa-kit","provider":"ABMIUM","version":"1.0","type":"link"}