{"product_id":"elk-elk5009-mouse-pdk4-pyruvate-dehydrogenase-kinase-isozyme-4-elisa-kit","title":"Mouse PDK4(Pyruvate Dehydrogenase Kinase Isozyme 4) ELISA Kit","description":"\u003cp\u003eMouse PDK4(Pyruvate Dehydrogenase Kinase Isozyme 4) ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of PDK4.\u003c\/p\u003e\u003ch3\u003eTest principle\u003c\/h3\u003e\u003cp\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PDK4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PDK4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PDK4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PDK4 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\u003ch3\u003eTarget background\u003c\/h3\u003e\u003cp\u003eAlternative names include [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial.\u003c\/p\u003e","brand":"ELK Biotechnology","offers":[{"title":"48T","offer_id":56181823013204,"sku":"ABM68768","price":284.4,"currency_code":"GBP","in_stock":true},{"title":"96T","offer_id":56181823045972,"sku":"ABM68767","price":407.05,"currency_code":"GBP","in_stock":true},{"title":"96T*5","offer_id":56181823078740,"sku":"ABM68769","price":1731.29,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/ELK5009-b-1.jpg?v=1781995786","url":"https:\/\/www.abmium.com\/products\/elk-elk5009-mouse-pdk4-pyruvate-dehydrogenase-kinase-isozyme-4-elisa-kit","provider":"ABMIUM","version":"1.0","type":"link"}