{"product_id":"elk-elk4734-human-pnf-h-phosphorylated-neurofilament-h-elisa-kit","title":"Human pNF-H(Phosphorylated Neurofilament H) ELISA Kit","description":"\u003cp\u003eHuman pNF-H(Phosphorylated Neurofilament H) ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of pNF-H.\u003c\/p\u003e\u003ch3\u003eTest principle\u003c\/h3\u003e\u003cp\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human pNF-H. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human pNF-H. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human pNF-H, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human pNF-H in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\u003ch3\u003eTarget background\u003c\/h3\u003e\u003cp\u003eAlternative names include NFH; 200 kDa neurofilament protein; Neurofilament triplet H protein; NEFH; Neurofilament, Heavy Polypeptide.\u003c\/p\u003e","brand":"ELK Biotechnology","offers":[{"title":"48T","offer_id":56181763899732,"sku":"ABM67823","price":284.4,"currency_code":"GBP","in_stock":true},{"title":"96T","offer_id":56181763932500,"sku":"ABM67822","price":407.05,"currency_code":"GBP","in_stock":true},{"title":"96T*5","offer_id":56181763965268,"sku":"ABM67824","price":1731.29,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/ELK4734-b-1.jpg?v=1781995220","url":"https:\/\/www.abmium.com\/products\/elk-elk4734-human-pnf-h-phosphorylated-neurofilament-h-elisa-kit","provider":"ABMIUM","version":"1.0","type":"link"}