{"product_id":"elk-elk3945-human-pde4d-phosphodiesterase-4d-camp-specific-elisa-kit","title":"Human PDE4D(Phosphodiesterase 4D, cAMP Specific) ELISA Kit","description":"\u003cp\u003eHuman PDE4D(Phosphodiesterase 4D, cAMP Specific) ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of PDE4D.\u003c\/p\u003e\u003ch3\u003eTest principle\u003c\/h3\u003e\u003cp\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PDE4D. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PDE4D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PDE4D, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PDE4D in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\u003ch3\u003eTarget background\u003c\/h3\u003e\u003cp\u003eAlternative names include DPDE3; PDE43; Dunce(Drosophila)Homolog Phosphodiesterase E3; cAMP-specific 3',5'-cyclic phosphodiesterase 4D.\u003c\/p\u003e","brand":"ELK Biotechnology","offers":[{"title":"48T","offer_id":56181485470036,"sku":"ABM65204","price":284.4,"currency_code":"GBP","in_stock":true},{"title":"96T","offer_id":56181485502804,"sku":"ABM65203","price":407.05,"currency_code":"GBP","in_stock":true},{"title":"96T*5","offer_id":56181485535572,"sku":"ABM65205","price":1731.29,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/ELK3945-b-1.jpg?v=1781993569","url":"https:\/\/www.abmium.com\/products\/elk-elk3945-human-pde4d-phosphodiesterase-4d-camp-specific-elisa-kit","provider":"ABMIUM","version":"1.0","type":"link"}