{"product_id":"elk-elk3886-human-nhe1-sodium-hydrogen-exchanger-1-elisa-kit","title":"Human NHE1(Sodium\/Hydrogen Exchanger 1) ELISA Kit","description":"\u003cp\u003eHuman NHE1(Sodium\/Hydrogen Exchanger 1) ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of NHE1.\u003c\/p\u003e\u003ch3\u003eTest principle\u003c\/h3\u003e\u003cp\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NHE1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NHE1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NHE1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NHE1 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\u003ch3\u003eTarget background\u003c\/h3\u003e\u003cp\u003eAlternative names include SLC9A1; APNH; Solute Carrier Family 9 Member 1,Sodium\/Hydrogen Exchanger; Antiporter,Na+\/H+,Amiloride Sensitive; Na(+)\/H(+) antiporter, amiloride-sensitive.\u003c\/p\u003e","brand":"ELK Biotechnology","offers":[{"title":"48T","offer_id":56181472067924,"sku":"ABM64982","price":284.4,"currency_code":"GBP","in_stock":true},{"title":"96T","offer_id":56181472100692,"sku":"ABM64981","price":407.05,"currency_code":"GBP","in_stock":true},{"title":"96T*5","offer_id":56181472133460,"sku":"ABM64983","price":1731.29,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/ELK3886-b-1.jpg?v=1781993449","url":"https:\/\/www.abmium.com\/products\/elk-elk3886-human-nhe1-sodium-hydrogen-exchanger-1-elisa-kit","provider":"ABMIUM","version":"1.0","type":"link"}