{"product_id":"elk-elk11056ms-human-e-cad-e-cadherin-microsample-elisa-kit","title":"Human E-Cad(E-Cadherin) Microsample ELISA Kit","description":"\u003cp\u003eHuman E-Cad(E-Cadherin) Microsample ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of E-Cad.\u003c\/p\u003e\u003ch3\u003eTest principle\u003c\/h3\u003e\u003cp\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human E-Cad. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human E-Cad. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human E-Cad, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human E-Cad in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\u003ch3\u003eTarget background\u003c\/h3\u003e\u003cp\u003eAlternative names include CD324; CDH1; Arc1; ECAD; CDH-E; E-CAD; LCAM; UVO; Cadherin 1 Type 1; Uvomorulin; E-Cadherin; Calcium-Dependent Adhesion Protein, Epithelial; Liver Cell Adhesion Molecule; Cadherin, Epithelial.\u003c\/p\u003e","brand":"ELK Biotechnology","offers":[{"title":"48T","offer_id":56180400357716,"sku":"ABM54623","price":236.41,"currency_code":"GBP","in_stock":true},{"title":"96T","offer_id":56180400390484,"sku":"ABM54622","price":334.17,"currency_code":"GBP","in_stock":true},{"title":"96T*5","offer_id":56180400423252,"sku":"ABM54624","price":1420.22,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/ELK11056MS-b-1.jpg?v=1781982685","url":"https:\/\/www.abmium.com\/products\/elk-elk11056ms-human-e-cad-e-cadherin-microsample-elisa-kit","provider":"ABMIUM","version":"1.0","type":"link"}