{"product_id":"elk-elk0928-hamster-tnf-a-tumor-necrosis-factor-alpha-elisa-kit","title":"Hamster TNF-a(Tumor Necrosis Factor-alpha) ELISA Kit","description":"\u003cp\u003eHamster TNF-a(Tumor Necrosis Factor-alpha) ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of TNF-a.\u003c\/p\u003e\u003ch3\u003eTest principle\u003c\/h3\u003e\u003cp\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hamster TNF-a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hamster TNF-a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hamster TNF-a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hamster TNF-a in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\u003ch3\u003eTarget background\u003c\/h3\u003e\u003cp\u003eAlternative names include DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2.\u003c\/p\u003e","brand":"ELK Biotechnology","offers":[{"title":"48T","offer_id":56180195230036,"sku":"ABM50285","price":236.41,"currency_code":"GBP","in_stock":true},{"title":"96T","offer_id":56180195262804,"sku":"ABM50284","price":334.17,"currency_code":"GBP","in_stock":true},{"title":"96T*5","offer_id":56180195295572,"sku":"ABM50286","price":1420.22,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/ELK0928-b-1.jpg?v=1781977845","url":"https:\/\/www.abmium.com\/products\/elk-elk0928-hamster-tnf-a-tumor-necrosis-factor-alpha-elisa-kit","provider":"ABMIUM","version":"1.0","type":"link"}