{"product_id":"elk-elk052es-easystep-mouse-25-oh-d-25-hydroxy-vitamin-d-elisa-kit","title":"EasyStep Mouse 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit","description":"\u003cp\u003eEasyStep Mouse 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of EasyStep Mouse 25-OH-D.\u003c\/p\u003e\u003ch3\u003eTest principle\u003c\/h3\u003e\u003cp\u003eThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse 25-OH-D antigen, and the Mouse 25-OH-D standard plate wells that pre-coated using protein-related techniques are provided separately. Standard\/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Mouse 25-OH-D to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Mouse 25-OH-D in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\u003ch3\u003eTarget background\u003c\/h3\u003e\u003cp\u003eAlternative names include 25 Hydroxy Vitamin D;.\u003c\/p\u003e","brand":"ELK Biotechnology","offers":[{"title":"48T","offer_id":56179973194068,"sku":"ABM49004","price":366.17,"currency_code":"GBP","in_stock":true},{"title":"96T","offer_id":56179973226836,"sku":"ABM49003","price":522.59,"currency_code":"GBP","in_stock":true},{"title":"96T*5","offer_id":56179973259604,"sku":"ABM49005","price":2221.88,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/ELK052ES-b-1.jpg?v=1781974516","url":"https:\/\/www.abmium.com\/products\/elk-elk052es-easystep-mouse-25-oh-d-25-hydroxy-vitamin-d-elisa-kit","provider":"ABMIUM","version":"1.0","type":"link"}