{"product_id":"elk-elk0113ms-human-f-actin-filamentous-actin-microsample-elisa-kit","title":"Human F-actin(Filamentous Actin) Microsample ELISA Kit","description":"\u003cp\u003eHuman F-actin(Filamentous Actin) Microsample ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of F-actin.\u003c\/p\u003e\u003ch3\u003eTest principle\u003c\/h3\u003e\u003cp\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human F-actin. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human F-actin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human F-actin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human F-actin in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\u003ch3\u003eTarget background\u003c\/h3\u003e\u003cp\u003eAlternative names include Fibrous Actin.\u003c\/p\u003e","brand":"ELK Biotechnology","offers":[{"title":"48T","offer_id":56179912507732,"sku":"ABM47729","price":284.4,"currency_code":"GBP","in_stock":true},{"title":"96T","offer_id":56179912540500,"sku":"ABM47728","price":407.05,"currency_code":"GBP","in_stock":true},{"title":"96T*5","offer_id":56179912573268,"sku":"ABM47730","price":1731.29,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/ELK0113MS-b-1.jpg?v=1781973332","url":"https:\/\/www.abmium.com\/products\/elk-elk0113ms-human-f-actin-filamentous-actin-microsample-elisa-kit","provider":"ABMIUM","version":"1.0","type":"link"}