{"product_id":"calibrated-high-molecular-weight-color-prestained-protein-marker-25-400-kda","title":"Calibrated High Molecular Weight Color Prestained Protein Marker (25–400 kDa)","description":"\u003csection\u003eA\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"highlight\"\u003ehigh-purity large molecule recombinant colour prestained protein marker\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003especifically designed for research experiments. Through scientific proportioning of 10 precise molecular weight proteins (25, 45, 72, 100, 130, 160, 200, 250, 300, 400kDa), it addresses the core pain points of traditional prestained markers \"low accuracy and poor stability\" providing key support for improved experimental efficiency.\u003c\/section\u003e\n\u003csection\u003e\u003c\/section\u003e\n\u003csection\u003e\n\u003cdiv class=\"data-card\"\u003eColor differentiation design: 72kDa (orange-red), 25kDa (green), others (blue), allowing real-time observation without staining, no need to pause electrophoresis to judge progress, quick confirmation of transfer efficiency after membrane transfer\u003c\/div\u003e\n\u003cdiv class=\"data-card\"\u003eAuthoritative calibration guarantee: Calibrated with imported brand non-prestained marker standards, molecular weight deviation is far below industry average, accuracy comparable to non-prestained markers\u003c\/div\u003e\n\u003cul class=\"list-paddingleft-2\"\u003e\n\u003cli\u003ePre-mixed 1× SDS-PAGE loading buffer, no boiling required\u003c\/li\u003e\n\u003cli\u003eSpecialized for large molecules: 25-400kDa suitable for most research large molecule proteins\u003c\/li\u003e\n\u003cli\u003eClear band performance: 5-10μL loading volume produces sharp bands, no diffusion or trailing after electrophoresis\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003csection\u003e\n\u003ctable style=\"width: 560px;\" class=\"spec-table\" width=\"-230\"\u003e\n\u003cthead\u003e\n\u003ctr class=\"firstRow\"\u003e\n\u003cth style=\"width: 177.047px;\" width=\"\"\u003eProduct Name\u003c\/th\u003e\n\u003cth style=\"width: 180.469px;\" width=\"\"\u003eSpecification 1 (50μL)\u003c\/th\u003e\n\u003cth style=\"width: 180.484px;\" width=\"\"\u003eSpecification 2 (250μL)\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 177.047px;\" rowspan=\"2\" width=\"200\"\u003eCalibrated High Molecular Weight Color Prestained Protein Marker (25-400kDa)\u003c\/td\u003e\n\u003ctd style=\"width: 180.469px;\" rowspan=\"2\" width=\"200\"\u003eSuitable for small batchpre-experiments (approx. 5-10 uses)\u003c\/td\u003e\n\u003ctd style=\"width: 180.484px;\" rowspan=\"2\" width=\"200\"\u003eSuitable for regular laboratories (approx. 25-50 uses)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003c\/section\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e1. High Molecular Weight Accuracy: Comprehensive Leadership Over Domestic and International Counterparts\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"data-card\"\u003e\u003cspan\u003eThis product's accuracy significantly surpasses international brands; while deviation at 72kDa is relatively large, all other bands perform equally or better than international brands.\u003c\/span\u003e\u003c\/div\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2. Buffer System Compatibility: Stable Migration Across Scenarios\u003c\/strong\u003e\u003c\/p\u003e\nValidated across multiple systems including Tris-Glycine buffer (conventional SDS-PAGE system), Tris-Tricine buffer (small molecule protein separation system), and Bis-Tris buffer (high-resolution separation system), this product maintains stable migration in all systems\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e3. Extreme Condition Stability: Handling Complex Experimental Environments\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"data-card\"\u003eRepeated freeze-thaw testing: 50 freeze-thaw cycles (-20℃→room temperature), no significant changes in band clarity or molecular weight migration, meeting laboratory scenarios requiring \"frequent access\"\u003c\/div\u003e\n\u003cdiv class=\"data-card\"\u003eHigh temperature stability testing: 7 days at 40℃ simulating transportation environment, no protein degradation or aggregation, band integrity maintained well, addressing \"summer transportation deterioration\" concernsRoom temperature storage testing: 30 days at 25℃ room temperature, no observed band changes, short-term forgetting to return to refrigerator does not affect use\u003c\/div\u003e\n\u003cdiv class=\"data-card\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv class=\"data-card\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003e4. Operational Convenience: Cost-Reducing, Efficiency-Enhancing Research Assistant\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul class=\"list-paddingleft-2\"\u003e\n\u003cli\u003eNo boiling required: Avoids traditional marker \"boiling denaturation\" step, reduces protein loss, and lowers operational risks\u003c\/li\u003e\n\u003cli\u003eNo dilution required: Pre-mixed 1× loading buffer, ready for direct loading, easy for beginners to use quickly\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003csection\u003e\n\u003cdiv class=\"scene-module\"\u003e\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eInstructions for Use\u003c\/strong\u003e\u003c\/p\u003e\n\u003col class=\"list-paddingleft-2\"\u003e\n\u003cli\u003eRemove product from -20℃ freezer, place in 4℃ refrigerator (10-20 minutes) or room temperature (5-8 minutes) for natural thawing,\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"highlight\"\u003estrictly avoid water bath heating or boiling\u003c\/span\u003e;\u003c\/li\u003e\n\u003cli\u003eAfter thawing, gently mix (recommend centrifugation at 4000-8000rpm for 30s before mixing);\u003c\/li\u003e\n\u003cli\u003eAspirate 5-10μL, slowly add to gel loading well, adjust loading volume appropriately according to gel properties and experimental habits;\u003c\/li\u003e\n\u003cli\u003eReturn unused marker to -20℃,\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"highlight\"\u003erecommend aliquoting into 20-50μL\/tube\u003c\/span\u003e.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePrecautions\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul class=\"list-paddingleft-2\"\u003e\n\u003cli\u003eThis product already contains loading buffer,\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"highlight\"\u003edo not heat or boil\u003c\/span\u003e;\u003c\/li\u003e\n\u003cli\u003eTo reduce marker degradation and contamination risks, recommend aliquoting and storing at -20℃;\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan class=\"highlight\"\u003eFor research use only\u003c\/span\u003e.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e","brand":"EnkiLife","offers":[{"title":"50μL","offer_id":56025563529556,"sku":"ABM55","price":129.0,"currency_code":"GBP","in_stock":true},{"title":"250μL","offer_id":56025563562324,"sku":"ABM55","price":295.0,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/image_1759819073_OBi2dkur.jpg?v=1780915878","url":"https:\/\/www.abmium.com\/products\/calibrated-high-molecular-weight-color-prestained-protein-marker-25-400-kda","provider":"ABMIUM","version":"1.0","type":"link"}