{"product_id":"abm82931-accoa-acetyl-coa-elisa-kit","title":"AcCoA(Acetyl CoA) ELISA Kit","description":"\u003cp\u003eAcCoA(Acetyl CoA) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring AcCoA in Cell lysates, Tissue Lysate samples from General. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.\u003c\/p\u003e\n\u003ch3\u003eTest principle\u003c\/h3\u003e\u003cp\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to AcCoA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to AcCoA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain AcCoA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of AcCoA in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\u003ch3\u003eTarget background\u003c\/h3\u003e\u003cp\u003eAlternative names include AcCoA.\u003c\/p\u003e","brand":"ELK Biotechnology","offers":[{"title":"48T","offer_id":56184684675412,"sku":"ABM82931","price":284.0,"currency_code":"GBP","in_stock":true},{"title":"96T","offer_id":56184684708180,"sku":"ABM82930","price":407.0,"currency_code":"GBP","in_stock":true},{"title":"96T*5","offer_id":56184684740948,"sku":"ABM82932","price":1731.0,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/ELK9575-b-1.jpg?v=1782024473","url":"https:\/\/www.abmium.com\/products\/abm82931-accoa-acetyl-coa-elisa-kit","provider":"ABMIUM","version":"1.0","type":"link"}