{"product_id":"abm78037-3-nt-3-nitrotyrosine-elisa-kit","title":"3-NT(3-Nitrotyrosine) ELISA Kit","description":"\u003cp\u003e3-NT(3-Nitrotyrosine) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring 3-NT in Serum, Plasma, Other biological fluids samples from General. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.\u003c\/p\u003e\n\u003ch3\u003eTest principle\u003c\/h3\u003e\u003cp\u003eThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 3-NT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 3-NT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 3-NT in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\u003ch3\u003eTarget background\u003c\/h3\u003e\u003cp\u003eAlternative names include 3-Nitro-L-Tyrosine; 3-Nitrotyrosine; Nitrotyrosine.\u003c\/p\u003e\u003csection data-abmium-seo-guidance=\"true\"\u003e\u003ch2\u003eHow to evaluate 3-NT(3-Nitrotyrosine) ELISA Kit\u003c\/h2\u003e\n\u003cp\u003eAssess this research-use product against the requirements of your experimental workflow before ordering.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eConfirm the supported sample matrices, assay principle and measurement range.\u003c\/li\u003e\n\u003cli\u003eReview sensitivity, precision, standard-curve and control requirements in the supplied protocol.\u003c\/li\u003e\n\u003cli\u003eCheck storage conditions, preparation steps and required laboratory equipment before starting.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eIf a specification or current document is not shown, contact ABMIUM scientific support for assistance before use.\u003c\/p\u003e\u003c\/section\u003e","brand":"ELK Biotechnology","offers":[{"title":"48T","offer_id":56183811834196,"sku":"ABM78038","price":236.0,"currency_code":"GBP","in_stock":true},{"title":"96T","offer_id":56183811866964,"sku":"ABM78037","price":334.0,"currency_code":"GBP","in_stock":true},{"title":"96T*5","offer_id":56183811899732,"sku":"ABM78039","price":1420.0,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/ELK7866-b-1.jpg?v=1782005554","url":"https:\/\/www.abmium.com\/products\/abm78037-3-nt-3-nitrotyrosine-elisa-kit","provider":"ABMIUM","version":"1.0","type":"link"}