{"product_id":"abm57368-rat-ppar-gamma-elisa-kit","title":"Rat PPAR-γ(Peroxisome Proliferator Activated Receptor Gamma) ELISA Kit","description":"\u003cp\u003eRat PPAR-γ(Peroxisome Proliferator Activated Receptor Gamma) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring PPAR-γ in Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernatant, Other biological fluids samples from Rat. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.\u003c\/p\u003e\n\u003ch3\u003eTest principle\u003c\/h3\u003e\u003cp\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PPAR-γ. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PPAR-γ. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PPAR-γ, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PPAR-γ in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\u003ch3\u003eTarget background\u003c\/h3\u003e\u003cp\u003eAlternative names include PPAR-G; PPARG1; PPARG2; NR1C3; Glitazone Receptor; Nuclear Receptor Subfamily 1 Group C Member 3; PPARg; Peroxisome Proliferator Activated Receptor Gamma.\u003c\/p\u003e","brand":"ELK Biotechnology","offers":[{"title":"48T","offer_id":56180579795284,"sku":"ABM57368","price":284.0,"currency_code":"GBP","in_stock":true},{"title":"96T","offer_id":56180579828052,"sku":"ABM57367","price":407.0,"currency_code":"GBP","in_stock":true},{"title":"96T*5","offer_id":56180579860820,"sku":"ABM57369","price":1731.0,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/ELK1632-b-1.jpg?v=1781984347","url":"https:\/\/www.abmium.com\/products\/abm57368-rat-ppar-gamma-elisa-kit","provider":"ABMIUM","version":"1.0","type":"link"}