{"product_id":"abm55424-human-vitamin-d-elisa-kit","title":"Human VD(Vitamin D) ELISA Kit","description":"\u003cp\u003eHuman VD(Vitamin D) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring VD in Serum, Plasma, Other biological fluids samples from Human. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.\u003c\/p\u003e\n\u003ch3\u003eTest principle\u003c\/h3\u003e\u003cp\u003eThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human VD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human VD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VD in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\u003ch3\u003eTarget background\u003c\/h3\u003e\u003cp\u003eAlternative names include VD; VD1; Lamisterol; Vitamin D; Vitamin D1; Lumisterol.\u003c\/p\u003e","brand":"ELK Biotechnology","offers":[{"title":"48T","offer_id":56180437713236,"sku":"ABM55424","price":236.0,"currency_code":"GBP","in_stock":true},{"title":"96T","offer_id":56180437746004,"sku":"ABM55423","price":334.0,"currency_code":"GBP","in_stock":true},{"title":"96T*5","offer_id":56180437778772,"sku":"ABM55425","price":1420.0,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/ELK11264-b-1.jpg?v=1781983340","url":"https:\/\/www.abmium.com\/products\/abm55424-human-vitamin-d-elisa-kit","provider":"ABMIUM","version":"1.0","type":"link"}