{"product_id":"abm-48245-piezo1-elisa-kit","title":"Mouse PIEZO-1(Piezo-Type mMechanosensitive iIon Channel Component 1) ELISA Kit","description":"\u003cp\u003eMouse PIEZO-1(Piezo-Type mMechanosensitive iIon Channel Component 1) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring PIEZO-1 in Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernatant, Other biological fluids samples from Mouse. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.\u003c\/p\u003e\n\u003ch3\u003eTest principle\u003c\/h3\u003e\u003cp\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PIEZO-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PIEZO-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PIEZO-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PIEZO-1 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\u003ch3\u003eTarget background\u003c\/h3\u003e\u003cp\u003eAlternative names include PIEZO1; FAM38A; KIAA0233.\u003c\/p\u003e","brand":"ELK Biotechnology","offers":[{"title":"48T","offer_id":56179938296148,"sku":"ABM48245","price":284.0,"currency_code":"GBP","in_stock":true},{"title":"96T","offer_id":56179938328916,"sku":"ABM48244","price":407.0,"currency_code":"GBP","in_stock":true},{"title":"96T*5","offer_id":56179938361684,"sku":"ABM48246","price":1731.0,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/ELK0282-b-1.jpg?v=1781973598","url":"https:\/\/www.abmium.com\/products\/abm-48245-piezo1-elisa-kit","provider":"ABMIUM","version":"1.0","type":"link"}