{"product_id":"abm-48977-25-oh-vitamin-d-easystep-elisa-kit","title":"EasyStep Human 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit","description":"\u003cp\u003eEasyStep Human 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring EasyStep Human 25-OH-D in Serum, Plasma samples from Human. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.\u003c\/p\u003e\n\u003ch3\u003eTest principle\u003c\/h3\u003e\u003cp\u003eThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 25-OH-D antigen, and the Human 25-OH-D standard plate wells that pre-coated using protein-related techniques are provided separately. Standard\/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human 25-OH-D to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Human 25-OH-D in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\u003ch3\u003eTarget background\u003c\/h3\u003e\u003cp\u003eAlternative names include 25 Hydroxy Vitamin D;.\u003c\/p\u003e","brand":"ELK Biotechnology","offers":[{"title":"48T","offer_id":56179972079956,"sku":"ABM48977","price":366.0,"currency_code":"GBP","in_stock":true},{"title":"96T","offer_id":56179972112724,"sku":"ABM48976","price":523.0,"currency_code":"GBP","in_stock":true},{"title":"96T*5","offer_id":56179972145492,"sku":"ABM48978","price":2222.0,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/ELK051ES-b-1.jpg?v=1781974599","url":"https:\/\/www.abmium.com\/nl\/products\/abm-48977-25-oh-vitamin-d-easystep-elisa-kit","provider":"ABMIUM","version":"1.0","type":"link"}