{"product_id":"elk-ept089-recombinant-rat-tnf-alpha","title":"Recombinant Rat TNF alpha","description":"\u003cp\u003eRecombinant Rat TNF alpha is a research-use recombinant protein from ELK Biotechnology preparation representing TNF alpha supplied as Lyophilized. Review the expression, purity, formulation and storage specifications below before selecting it for an assay or functional workflow.\u003c\/p\u003e\n\u003ch3\u003eDescription\u003c\/h3\u003e\u003cp\u003eRecombinant Rat Tumor Necrosis Factor is produced by our E.coli expression system and the target gene encoding Leu80-Leu235 is expressed.\u003c\/p\u003e\u003ch3\u003eBackground\u003c\/h3\u003e\u003cp\u003eTumor necrosis factor alpha (TNF-alpha, TNFSF2) is the prototypic ligand of the TNF superfamily. Rat TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 179 aa extracellular domain (ECD). Within the ECD, rat TNF-alpha shares 94% aa sequence identity with mouse. TNF-alpha is produced by a wide variety of immune, epithelial, endothelial, and tumor cells. TNF exists as a homotrimer and interacts with SPPL2B. TNF is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. TNF is a key cytokine in the development of several inflammatory disorders. It contributes to the development of type 2 diabetes throught its effects on insulin resistance and fatty acid metabolism.\u003c\/p\u003e\u003ch3\u003eHandling information\u003c\/h3\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eReconstitution\u003c\/th\u003e\n\u003ctd\u003eAlways centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100µg\/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDelivery condition\u003c\/th\u003e\n\u003ctd\u003eThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e","brand":"ELK Biotechnology","offers":[{"title":"50ug","offer_id":56187602272596,"sku":"ABM100813","price":523.0,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/3_9bac09ba-4746-416e-a453-0758842d3947.png?v=1783151425","url":"https:\/\/www.abmium.com\/ja\/products\/elk-ept089-recombinant-rat-tnf-alpha","provider":"ABMIUM","version":"1.0","type":"link"}