{"product_id":"elk-elk5712-guinea-pig-et-1-endothelin1-elisa-kit","title":"Guinea pig ET-1(Endothelin1) ELISA Kit","description":"\u003cp\u003eGuinea pig ET-1(Endothelin1) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring ET-1 in Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernatant, Other biological fluids samples from Guinea pig. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.\u003c\/p\u003e\n\u003ch3\u003eTest principle\u003c\/h3\u003e\u003cp\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Guinea pig ET-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Guinea pig ET-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Guinea pig ET-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Guinea pig ET-1 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\u003ch3\u003eTarget background\u003c\/h3\u003e\u003cp\u003eAlternative names include ET1; PPET1; Preproendothelin-1; Big endothelin-1; EDN1.\u003c\/p\u003e","brand":"ELK Biotechnology","offers":[{"title":"48T","offer_id":56182579986772,"sku":"ABM70850","price":366.0,"currency_code":"GBP","in_stock":true},{"title":"96T","offer_id":56182580019540,"sku":"ABM70849","price":523.0,"currency_code":"GBP","in_stock":true},{"title":"96T*5","offer_id":56182580052308,"sku":"ABM70851","price":2222.0,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/ELK5712-b-1.jpg?v=1781997793","url":"https:\/\/www.abmium.com\/ja\/products\/elk-elk5712-guinea-pig-et-1-endothelin1-elisa-kit","provider":"ABMIUM","version":"1.0","type":"link"}