{"product_id":"anti-lrrc75a-antibody-picoband-a18461-1","title":"Anti-LRRC75A Antibody Picoband®","description":"\u003cp\u003eAnti-LRRC75A Antibody Picoband® is a research-use antibody from Boster Bio for studying Leucine-rich repeat-containing protein 75A in ELISA, Flow Cytometry, IHC, IHC-P, WB workflows with listed reactivity for Human. Specifications and supporting documents below should be reviewed when planning the experiment.\u003c\/p\u003e\nBoster Bio Anti-LRRC75A Antibody Picoband® catalog # A18461-1. Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.\u003cp\u003eLong noncoding RNAs (lncRNAs) play multiple key roles during inflammatory processes. A novel lncRNA identified by the high-throughput sequencing analysis was found significantly down-regulated in Escherichia coli-introduced cell model of bovine mastitis. Given that this lncRNA consists of the antisense of leucine-rich repeat-containing protein 75A (LRRC75A), it was named LRRC75A antisense lncRNA1 (LRRC75A-AS1). The expression of LRRC75A-AS1 was down-regulated in bovine mammary epithelial cells and mammary tissues under inflammatory condition. Knockout (KO) of LRRC75A-AS1 by CRISPR-Cas9 system in bovine mammary alveolar cell-T (MAC-T) cell line could enhance expressions of tight junction (TJ) proteins Claudin-1, Occludin and ZO-1, reduce cell monolayer permeability, and inhibit Staphylococcus aureus adhesion and invasion. Meanwhile, it also down-regulated expressions of inflammatory factors and attenuated activation of NF-κB pathway. Similarly, knockdown of LRRC75A caused the changes as LRRC75A-AS1 KO did, while overexpression of LRRC75A enabled the opposite effects. TJ of epithelioid cells barriers the pathogenic microorganisms outside during inflammation, in which LRRC75A-AS1 can regulate the expression of TJ proteins through LRRC75A, affecting the development of inflammation.\u003c\/p\u003e\u003ch3\u003eProduct information\u003c\/h3\u003e","brand":"Boster Bio","offers":[{"title":"Unconjugated \/ 100 μg\/vial","offer_id":56042214162772,"sku":"ABM26079-1","price":328.0,"currency_code":"GBP","in_stock":true},{"title":"Biotin \/ 100 μg\/vial","offer_id":56042214195540,"sku":"ABM26079-2","price":505.0,"currency_code":"GBP","in_stock":true},{"title":"Cy3 \/ 100 μg\/vial","offer_id":56042214228308,"sku":"ABM26079-3","price":505.0,"currency_code":"GBP","in_stock":true},{"title":"Fluoro488 \/ 100 μg\/vial","offer_id":56042214261076,"sku":"ABM26079-4","price":505.0,"currency_code":"GBP","in_stock":true},{"title":"Fluoro550 \/ 100 μg\/vial","offer_id":56042214293844,"sku":"ABM26079-5","price":505.0,"currency_code":"GBP","in_stock":true},{"title":"Fluoro594 \/ 100 μg\/vial","offer_id":56042214326612,"sku":"ABM26079-6","price":505.0,"currency_code":"GBP","in_stock":true},{"title":"FITC \/ 100 μg\/vial","offer_id":56042214359380,"sku":"ABM26079-7","price":505.0,"currency_code":"GBP","in_stock":true},{"title":"HRP \/ 100 μg\/vial","offer_id":56042214392148,"sku":"ABM26079-8","price":505.0,"currency_code":"GBP","in_stock":true},{"title":"APC \/ 100 μg\/vial","offer_id":56042214424916,"sku":"ABM26079-9","price":726.0,"currency_code":"GBP","in_stock":true},{"title":"PE \/ 100 μg\/vial","offer_id":56042214457684,"sku":"ABM26079-10","price":726.0,"currency_code":"GBP","in_stock":true},{"title":"Fluoro647 \/ 100 μg\/vial","offer_id":56042214490452,"sku":"ABM26079-11","price":593.0,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/a18461-1-lrrc75a-primary-antibodies-wb-testing-1.jpg?v=1781654041","url":"https:\/\/www.abmium.com\/ja\/products\/anti-lrrc75a-antibody-picoband-a18461-1","provider":"ABMIUM","version":"1.0","type":"link"}