{"product_id":"proteogenix-human-igg1-l234a-l235a-kappa-isotype-control-antibody-hyhel-10-fitc","title":"Human IgG1 (L234A\/L235A), kappa Isotype Control Antibody (HyHEL-10), FITC","description":"\u003ch2\u003eHuman IgG1 (L234A\/L235A), kappa Isotype Control Antibody (HyHEL-10), FITC\u003c\/h2\u003e \u003ch2\u003eBiological Function\u003c\/h2\u003e \u003cp\u003eHuman IgG1 (L234A\/L235A), kappa Isotype Control Antibody (HyHEL-10), FITC is a monoclonal antibody that specifically binds to the kappa light chain of human immunoglobulin G1 (IgG1). This antibody has been engineered to contain two amino acid mutations (L234A\/L235A) that enhance its binding affinity and specificity.\u003c\/p\u003e \u003ch2\u003eMain Applications\u003c\/h2\u003e \u003cp\u003eThis antibody is commonly used as an isotype control in flow cytometry experiments to distinguish between specific and non-specific binding of antibodies. It can also be used in western blotting and immunohistochemistry applications to ensure the specificity of target antibody binding.\u003c\/p\u003e \u003ch2\u003eExperimental Use Cases\u003c\/h2\u003e \u003cp\u003eIn flow cytometry experiments, Human IgG1 (L234A\/L235A), kappa Isotype Control Antibody (HyHEL-10), FITC is used as a negative control to assess the background fluorescence of cells and to determine the appropriate gating strategy. It is also used to confirm the specificity of target antibody binding by comparing the signal intensity between the isotype control and the target antibody.\u003c\/p\u003e \u003cp\u003eIn western blotting, this antibody is used as a negative control to ensure that the observed bands are specific to the target protein and not due to non-specific binding. It can also be used in immunohistochemistry to confirm the specificity of target antibody binding and to assess the level of non-specific background staining.\u003c\/p\u003e \u003ch2\u003e\u003c\/h2\u003e \u003cp\u003eThis Human IgG1 (L234A\/L235A), kappa Isotype Control Antibody (HyHEL-10), FITC is a highly specific and sensitive tool for flow cytometry, western blotting, and immunohistochemistry experiments. Its enhanced binding affinity and specificity make it an ideal choice for researchers looking to accurately distinguish between specific and non-specific antibody binding.\u003c\/p\u003e \u003ch2\u003eStructure\u003c\/h2\u003e \u003cp\u003eTo summarize, Human IgG1 (L234A\/L235A), kappa Isotype Control Antibody (HyHEL-10), FITC is a monoclonal antibody with two amino acid mutations that enhance its binding affinity and specificity for the kappa light chain of human IgG1. It is commonly used as an isotype control in flow cytometry, western blotting, and immunohistochemistry experiments to ensure the specificity of target antibody binding. This antibody is a valuable tool for researchers looking to accurately assess and control for non-specific binding in their experiments.\u003c\/p\u003e\u003cp\u003eProteoGenix catalogue number ARO-A23186. For research use only.\u003c\/p\u003e\u003cp\u003eTarget: Human IgG1 (L234A \/L235A), kappa; Clonality: Monoclonal Antibody; Applications: Flow Cyt.\u003c\/p\u003e\u003cp\u003eAvailable configurations: 50ug, 100ug, 1mg.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/www.proteogenix.science\/wp-content\/uploads\/documents\/Datasheets\/ARO-A23186.pdf\"\u003eDownload the manufacturer datasheet\u003c\/a\u003e\u003c\/p\u003e","brand":"ProteoGenix","offers":[{"title":"50ug","offer_id":57357936558420,"sku":"ABM135032-1","price":191.2,"currency_code":"GBP","in_stock":true},{"title":"100ug","offer_id":57357936591188,"sku":"ABM135032-2","price":263.2,"currency_code":"GBP","in_stock":true},{"title":"1mg","offer_id":57357936623956,"sku":"ABM135032-3","price":903.2,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/Primaryantibodies-CCt9H6Tc_7472664b-8efa-40e1-a9e8-0e8ccc929dc7.svg?v=1790891373","url":"https:\/\/www.abmium.com\/es\/products\/proteogenix-human-igg1-l234a-l235a-kappa-isotype-control-antibody-hyhel-10-fitc","provider":"ABMIUM","version":"1.0","type":"link"}