{"product_id":"p21-cdkn1a-recombinant-rabbit-monoclonal-antibody-amre86606","title":"p21 \/ CDKN1A Recombinant Rabbit Monoclonal Antibody","description":"\u003cp\u003eRecombinant rabbit monoclonal antibody recognising human p21 (CDKN1A), a cyclin-dependent kinase inhibitor relevant to senescence, proliferation and p53-dependent checkpoint signalling.\u003c\/p\u003e\u003ch3\u003eTarget background\u003c\/h3\u003e\u003cp\u003ep21 is an inhibitor of cyclin-dependent kinase complexes, particularly those involving CDK2 and CDK4, and helps prevent progression from G1 into S phase. TP53 can induce CDKN1A transcription after cellular stress, making p21 a central effector of p53-mediated cell-cycle arrest. The protein can also interact with proliferating cell nuclear antigen (PCNA) and contribute to regulation of DNA replication and repair. p21 has been implicated in apoptosis and cellular senescence, and the CDKN1A gene produces alternative transcript forms.\u003c\/p\u003e\u003ch3\u003eAlso known as\u003c\/h3\u003e\u003cp\u003eCDKN1A, p21, p21CIP1, CIP1, WAF1, SDI1, CAP20, CDKN1, MDA-6; also p21\/WAF1\/CIP1.\u003c\/p\u003e\u003ch3\u003eManufacturer specifications\u003c\/h3\u003e\u003cul\u003e\n\u003cli\u003eHost: rabbit; recombinant monoclonal IgG; unconjugated. Reactivity: human.\u003c\/li\u003e\n\u003cli\u003eManufacturer applications: WB 1:1,000–1:5,000; IHC 1:100–1:200; ICC\/IF 1:500–1:1,000; flow cytometry 1:50–1:200; IP 1:10–1:100.\u003c\/li\u003e\n\u003cli\u003ePurification: affinity purification; form: liquid; immunogen: synthetic peptide of human p21.\u003c\/li\u003e\n\u003cli\u003eCalculated molecular weight: 18 kDa; observed molecular weight: 21 kDa. Gene ID: 1026; UniProtKB: P38936.\u003c\/li\u003e\n\u003cli\u003eBuffer: 50 mM Tris-glycine pH 7.4, 0.15 M NaCl, 40% glycerol, 0.01% sodium azide and 0.05% protective protein. Short-term storage at 4 °C; aliquot and keep at −20 °C longer term.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch3\u003eManufacturer application evidence\u003c\/h3\u003e\u003cp\u003eManufacturer western blot evidence in human HeLa and HCT116 lysates at 1:1,000. The additional manufacturer-listed applications have not been individually image-supported in the provided datasheet.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProvenance:\u003c\/strong\u003e Original EnkiLife antibody. Manufacturer-supplied data; no claim of ABMIUM independent validation. Research use only. \u003ca href=\"https:\/\/www.enkilife.com\/p\/p21-Rabbit-Monoclonal-Antibody-AMRe86606\"\u003eManufacturer product page\u003c\/a\u003e · \u003ca href=\"https:\/\/assets.enkilife.com\/files\/recombinant_antibody\/AMRe86606_EnkiLife_EN.pdf\"\u003eProduct datasheet (PDF)\u003c\/a\u003e.\u003c\/p\u003e","brand":"EnkiLife","offers":[{"title":"20 µL","offer_id":57476185063764,"sku":"ABM149205-2","price":58.65,"currency_code":"GBP","in_stock":true},{"title":"50 µL","offer_id":57476185096532,"sku":"ABM149205-3","price":101.15,"currency_code":"GBP","in_stock":true},{"title":"100 µL","offer_id":57474778399060,"sku":"ABM149205-1","price":177.65,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/AMRe86606_202.jpg?v=1791450350","url":"https:\/\/www.abmium.com\/de\/products\/p21-cdkn1a-recombinant-rabbit-monoclonal-antibody-amre86606","provider":"ABMIUM","version":"1.0","type":"link"}