{"product_id":"elk-es1089-histone-h3-acetyl-lys23-rabbit-pab","title":"Histone H3 (Acetyl Lys23) rabbit pAb","description":"\u003cp\u003eHistone H3 (Acetyl Lys23) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Histone H3 in WB, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.\u003c\/p\u003e\n\u003ch3\u003eBackground\u003c\/h3\u003e\u003cp\u003eHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015],\u003c\/p\u003e\u003ch3\u003eAdditional antibody information\u003c\/h3\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecificity\u003c\/th\u003e\n\u003ctd\u003eAcetyl-Histone H3 (K23) Polyclonal Antibody detects endogenous levels of Histone H3 protein only when acetylated at K23.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eValidation evidence captions\u003c\/th\u003e\n\u003ctd\u003eWestern Blot analysis of various cells using Acetyl-Histone H3 (K23) Polyclonal Antibody diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Western Blot analysis of L929 cells using Acetyl-Histone H3 (K23) Polyclonal Antibody diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunofluorescence analysis of HeLa cells, using Histone H3 (Acetyl-Lys23) Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from Raw264.7 cells, treated with TSA 400nM 24h, using Histone H3 (Acetyl-Lys23) Antibody. The lane on the right is blocked with the synthesized peptide.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e","brand":"ELK Biotechnology","offers":[{"title":"Unconjugated \/ 100µL","offer_id":56184977916244,"sku":"ABM102966","price":220.0,"currency_code":"GBP","in_stock":true},{"title":"Unconjugated \/ 50µL","offer_id":56184977949012,"sku":"ABM93102","price":132.0,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/ES1089-c-1.jpg?v=1782028948","url":"https:\/\/www.abmium.com\/da\/products\/elk-es1089-histone-h3-acetyl-lys23-rabbit-pab","provider":"ABMIUM","version":"1.0","type":"link"}