{"product_id":"anti-b3gnt8-antibody-picoband-pb9686","title":"Anti-B3GNT8 Antibody Picoband®","description":"\u003cp\u003eAnti-B3GNT8 Antibody Picoband® is a research-use antibody from Boster Bio for studying UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 8 in ELISA, Flow Cytometry, IHC, IHC-P, WB workflows with listed reactivity for Human. Specifications and supporting documents below should be reviewed when planning the experiment.\u003c\/p\u003e\nBoster Bio Anti-B3GNT8 Antibody Picoband® catalog # PB9686. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.\u003cp\u003eB3GNT8 is a galactosyltransferase involved in the synthesis of poly-N-acetyllactosamine (polyLacNAc), a linear chain of repeating LacNAc units made up of galactose (Gal) and N-acetylglucosamine (GlcNAc) with the structure (Gal-beta-1-4-GlcNAc-beta-1-3)n. By genomic sequence analysis, the B3GNT8 gene is mapped to chromosome 19q13.2. It was showed that a soluble form of B3GNT8 overexpressed by transfected HEK293 cells selectively transferred GlcNAc from UDP-GlcNAc to the nonreducing terminus of Gal-beta-1-4-GlcNAc-alpha-p-nitrophenyl phosphate and to lactoside-alpha-benzoyl. It did not utilize keratan sulfates or polylactosamine oligosaccharide as substrate. B3GNT8 activity required Mn (2+) and showed less efficiency with Co (2+). The pH optimum was between 7 and 7.5. B3GNT8 also transferred GlcNAc onto alpha-1-acid glycoprotein and ovomucoid, which possess tetraantennary complex type and pentaantennary complex type N-glycans. With a tetraantennary N-glycan substrate, B3GNT8 appeared to prefer the beta-1-2 branch over the beta-1-6 branch. When overexpressed in HCT15 human colon cancer cells, B3GNT8 increased cell surface expression of both polyLacNAc and beta-1-6-branched N-glycans.\u003c\/p\u003e\u003ch3\u003eProduct information\u003c\/h3\u003e","brand":"Boster Bio","offers":[{"title":"Unconjugated \/ 100 μg\/vial","offer_id":56039404798292,"sku":"ABM21151-1","price":328.0,"currency_code":"GBP","in_stock":true},{"title":"Biotin \/ 100 μg\/vial","offer_id":56039404831060,"sku":"ABM21151-2","price":505.0,"currency_code":"GBP","in_stock":true},{"title":"Cy3 \/ 100 μg\/vial","offer_id":56039404863828,"sku":"ABM21151-3","price":505.0,"currency_code":"GBP","in_stock":true},{"title":"Fluoro488 \/ 100 μg\/vial","offer_id":56039404896596,"sku":"ABM21151-4","price":505.0,"currency_code":"GBP","in_stock":true},{"title":"Fluoro550 \/ 100 μg\/vial","offer_id":56039404929364,"sku":"ABM21151-5","price":505.0,"currency_code":"GBP","in_stock":true},{"title":"Fluoro594 \/ 100 μg\/vial","offer_id":56039404962132,"sku":"ABM21151-6","price":505.0,"currency_code":"GBP","in_stock":true},{"title":"FITC \/ 100 μg\/vial","offer_id":56039404994900,"sku":"ABM21151-7","price":505.0,"currency_code":"GBP","in_stock":true},{"title":"HRP \/ 100 μg\/vial","offer_id":56039405027668,"sku":"ABM21151-8","price":505.0,"currency_code":"GBP","in_stock":true},{"title":"APC \/ 100 μg\/vial","offer_id":56039405060436,"sku":"ABM21151-9","price":726.0,"currency_code":"GBP","in_stock":true},{"title":"PE \/ 100 μg\/vial","offer_id":56039405093204,"sku":"ABM21151-10","price":726.0,"currency_code":"GBP","in_stock":true},{"title":"Fluoro647 \/ 100 μg\/vial","offer_id":56039405125972,"sku":"ABM21151-11","price":593.0,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/pb9686-1-WB-anti-b3gnt8-picoband-antibody.jpg?v=1781635017","url":"https:\/\/www.abmium.com\/da\/products\/anti-b3gnt8-antibody-picoband-pb9686","provider":"ABMIUM","version":"1.0","type":"link"}