{"product_id":"abm55325-rat-ca15-3-elisa-kit","title":"Rat CA15-3(Carbonhydrate Antigen 15-3) ELISA Kit","description":"\u003cp\u003eRat CA15-3(Carbonhydrate Antigen 15-3) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring CA15-3 in Serum, Plasma, Tissue homogenates, Cell lysates, Other biological fluids samples from Rat. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.\u003c\/p\u003e\n\u003ch3\u003eTest principle\u003c\/h3\u003e\u003cp\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CA15-3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CA15-3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CA15-3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CA15-3 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\u003ch3\u003eTarget background\u003c\/h3\u003e\u003cp\u003eAlternative names include CA15-3.\u003c\/p\u003e","brand":"ELK Biotechnology","offers":[{"title":"48T","offer_id":56180433158484,"sku":"ABM55325","price":366.0,"currency_code":"GBP","in_stock":true},{"title":"96T","offer_id":56180433191252,"sku":"ABM55324","price":523.0,"currency_code":"GBP","in_stock":true},{"title":"96T*5","offer_id":56180433224020,"sku":"ABM55326","price":2222.0,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/ELK11235-b-1.jpg?v=1781983323","url":"https:\/\/www.abmium.com\/da\/products\/abm55325-rat-ca15-3-elisa-kit","provider":"ABMIUM","version":"1.0","type":"link"}