{"product_id":"abm49523-human-25-oh-d-elisa-kit","title":"Human 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit","description":"\u003cp\u003eHuman 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring 25-OH-D in Serum, Plasma, Other biological fluids samples from Human. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.\u003c\/p\u003e\n\u003ch3\u003eTest principle\u003c\/h3\u003e\u003cp\u003eThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 25-OH-D. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 25-OH-D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 25-OH-D in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\u003ch3\u003eTarget background\u003c\/h3\u003e\u003cp\u003eAlternative names include 25 Hydroxy Vitamin D;.\u003c\/p\u003e","brand":"ELK Biotechnology","offers":[{"title":"48T","offer_id":56180008059220,"sku":"ABM49523","price":236.0,"currency_code":"GBP","in_stock":true},{"title":"96T","offer_id":56180008091988,"sku":"ABM49522","price":334.0,"currency_code":"GBP","in_stock":true},{"title":"96T*5","offer_id":56180008124756,"sku":"ABM49524","price":1420.0,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/ELK0694-b-1.jpg?v=1781974933","url":"https:\/\/www.abmium.com\/cy\/products\/abm49523-human-25-oh-d-elisa-kit","provider":"ABMIUM","version":"1.0","type":"link"}